|
|
|
| Registros recuperados: 24 | |
|
| |
|
|
| Alves,Andrea Catão; Gontijo,Cristiano Machado; Oliveira,Mônica Cristina; Diniz,Simone Odília Fernandes; Oliveira,Flávia Márcia; Cardoso,Valbert Nascimento; Ramaldes,Gilson Andrade. |
| The oral administration of proteic antigens, like ovalbumin, may result in the induction of oral tolerance or immunization. The aim of this work was to label a protein antigen with 99mTechnetium, encapsulate it in liposomes and investigate its absorption and tissue distribution after oral administration in mice. Ovalbumin was labeled with 99mTechnetium and encapsulated in small unilamellar vesicles. 99mTc-OVA encapsulated or not in liposomes was administrated to mice that were sacrificed after different times. The radioactivity was measured in various organs of the animals. Differences concerning the biodistribution of 99mTc-OVA were noticed. The technique may represent alternatives for the induction of immunization or oral tolerance. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Liposomes; Oral absorption; Mucosal delivery; Proteins; Nanotechnology. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700035 |
| |
|
|
| Yuldasheva,L.N.; Carvalho,E.B.; Catanho,M.-T.J.A.; Krasilnikov,O.V.. |
| Plants used in traditional medicine are rich sources of hemolysins and cytolysins, which are potential bactericidal and anticancer drugs. The present study demonstrates for the first time the presence of a hemolysin in the leaves of Passiflora quadrangularis L. This hemolysin is heat stable, resistant to trypsin treatment, has the capacity to froth, and acts very rapidly. The hemolysin activity is dose-dependent, with a slope greater than 1 in a double-logarithmic plot. Polyethylene glycols of high molecular weight were able to reduce the rate of hemolysis, while liposomes containing cholesterol completely inhibited it. In contrast, liposomes containing phosphatidylcholine were ineffective. The Passiflora hemolysin markedly increased the conductance of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Passiflora quadrangularis; Leaf extract; Liposomes; Bilayer permeation; Hemolysin; Membrane cholesterol. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700009 |
| |
|
| |
|
|
| CIRINO,LORENA M.D.; VERGNE,DAVIANE M.C.; SANTANA,PATRÍCIA F.; ALMEIDA,ENRIK DE; COSTA,LUIZ P. DA; ALBUQUERQUE-JÚNIOR,RICARDO L.C. DE; LIMA-VERDE,ISABEL B.; PADILHA,FRANCINE F.; CARDOSO,JULIANA C.. |
| ABSTRACT The objective of this work was to study the reduction in the capsaicin toxicity by encapsulation in liposomes. Capsaicin was extracted from peppers and characterized with high performance liquid chromatography (HPLC). We determined the zeta potential, polydispersivity index (PdI) and vesicle size of liposomes. Wistar rats were submitted to intravesical instillation of liposomes (LIP), capsaicin (CAP) or liposomes with capsaicin (CAPLIP). After 24 hours, bladders were removed for histological analysis. Vesicle size ranged from 68 to 105 nm with PdI smaller than 0.2 and zeta potential around -30 mV. The vesicles maintained stability over the 14-day study. The histological analysis of the CAP group showed intense inflammation in almost all bladder... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Capsaicin; Liposomes; Urinary incontinence; Intravesical administration. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401539 |
| |
|
|
| Schettini,D.A.; Costa Val,A.P.; Souza,L.F.; Demicheli,C.; Rocha,O.G.F.; Melo,M.N.; Michalick,M.S.M.; Frézard,F.. |
| The achievement of complete cure in dogs with visceral leishmaniasis is currently a great challenge, since dogs are the main reservoir for the transmission of visceral leishmaniasis to humans and they respond poorly to conventional treatment with pentavalent antimonials. In order to improve the efficacy of treatment, we developed a novel formulation for meglumine antimoniate based on the encapsulation of this drug in freeze-dried liposomes (LMA). The aim of the present study was to evaluate the biodistribution of antimony (Sb) in dogs following a single intravenous bolus injection of LMA. Four healthy male mongrel dogs received LMA at 3.8 mg Sb/kg body weight and were sacrificed 3, 48 and 96 h and 7 days later. Antimony was determined in the blood, liver,... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Liposomes; Meglumine antimoniate; Biodistribution; Leishmaniasis; Dogs. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200015 |
| |
|
|
| Araújo,R.V.S.; Melo-Júnior,M.R.; Beltrão,E.I.C.; Mello,L.A.; Iacomini,M.; Carneiro-Leão,A.M.A.; Carvalho Jr,L.B.; Santos-Magalhães,N.S.. |
| The antischistosomal activity of the sulfated polysaccharide α-D-glucan (Glu.SO4) extracted from Ramalina celastri was evaluated after encapsulation into liposomes (Glu.SO4-LIPO) in Schistosoma mansoni-infected mice. The effect of treatment with Glu.SO4 and Glu.SO4-LIPO (10 mg/kg) on egg elimination, worm burden and hepatic granuloma formation was assessed using female albino Swiss mice, 35-40 days of age, weighing 25 ± 2 g, infected with 150 cercariae/animal (Biomphalaria glabrata, BH strain). Four groups (N = 10) were studied, two controls (empty liposomes and NaCl) and two treated groups (Glu.SO4-LIPO and Glu.SO4) using a single dose. Parasitological analysis revealed that Glu.SO4-LIPO was as efficient as Glu.SO4 in reducing egg elimination and worm... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polysaccharide; Α-D-glucan; Ramalina celastri; Liposomes; Schistosoma mansoni; Lectin histochemistry. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400007 |
| |
|
| |
|
|
| The possibility of producing neutralizing antibodies against the lethal effects of scorpion toxins was evaluated in the mouse model by immunization with an immunogen devoid of toxicity. A toxic fraction (5 mg) from the venom of the scorpion Tityus serrulatus was entrapped in sphingomyelin-cholesterol liposomes. The liposomes were treated for 1 h at 37oC with a 1% (w/w) trypsin solution in 0.2 M sodium carbonate buffer, pH 8.3. This treatment led to a strong reduction in venom toxicity. Immunization was performed as follows: mice were injected sc with 20 µg of the liposome-entrapped toxic fraction on days 1 and 21 and a final injection (20 µg) was administered ip on day 36. After injection of the immunogen, all mice developed an IgG response which was shown... |
|
Palavras-chave: Liposomes; Neutralizing antibodies; Tityus serrulatus; Immunization; Toxins. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700010 |
| |
|
|
| Cavalcanti,Isabella Macário Ferro; Menezes,Talita Gomes Calaça; Campos,Luís André de Almeida; Ferraz,Milena Sales; Maciel,Maria Amélia Vieira; Caetano,Maria Nelly Psiotano; Santos-Magalhães,Nereide Stela. |
| ABSTRACT The treatment of infections caused by resistant microorganisms is limited, and vancomycin (VAN) treatment failures for methicillin-resistant Staphylococcus aureus (MRSA) bacteremia are not uncommon, even when MRSA clinical isolates are susceptible to VAN. Thus, this study proposed the association of VAN with usnic acid and β-lapachone encapsulated into liposomes as a novel therapeutic option for infections caused by MRSA. Liposomes containing β-lap (β-lap-lipo) or usnic acid (UA-lipo) were prepared by the thin lipid film hydration method followed by sonication. Antimicrobial activity against MRSA clinical isolates was investigated by the microdilution method according to the Clinical and Laboratory Standards Institute (CLSI). The interaction... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Β-lapachone; Usnic acid; Liposomes; Methicillin-resistant Staphylococcus aureus (MRSA); Synergism.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200627 |
| |
|
| |
|
|
| CAMILO,Cicera J.; LEITE,Débora O. D.; SILVA,Angelo R. A. e; MENEZES,Irwin R. A.; COUTINHO,Henrique D. M.; COSTA,José G. M. da. |
| ABSTRACT Liposomes and niosomes are currently the most studied lipid vesicles in the nanomedicine field. The system formed by a phospholipid bilayer in aqueous medium allows these vesicles to carry both hydrophilic and lipophilic compounds, providing an increase in solubility of drugs already used in conventional therapy. The focus on the development of these vesicles should be directed to determining the ideal composition, with low toxicity, biocompatibility and which remains stable for long periods. These characteristics are related to the components used for formulation and the substances that will be encapsulated. Another important point relates to the methods used during formulation, which are important in determining the type of vesicle formed,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Components; Formulation; Liposomes; Nanomedicine; Niosomes. |
| Ano: 2020 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2020000200339 |
| |
|
| |
|
|
| El-Nakeeb,Moustafa A; Abou-Shleib,Hamida M; Khalil,Amal M; Omar,Hoda G; El-Halfawy,Omar M. |
| Several antihistaminics possess antibacterial activity against a broad spectrum of bacteria. However, the exact mechanism of such activity was unclear. Hence, the aim of this study is to investigate their mechanism of antibacterial activity especially their effect upon the permeability of the bacterial cytoplasmic membrane. The effects of azelastine, cetirizine, cyproheptadine and diphenhydramine were studied using Gram-positive and Gram-negative multiresistant clinical isolates. Leakage of 260 and 280 nm UV-absorbing materials was detected upon treatment with the tested antihistaminics; indicative of membrane alteration. Using an artificial membrane model, cholesterol-free negatively-charged unilamellar liposomes, confirmed the effect of antihistaminics... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antihistaminics; Azelastine; Bacteria; Cytoplasmic membrane; Liposomes; Permeability alteration. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300019 |
| |
|
|
| Volpedo,Greta; Costa,Lourena; Ryan,Nathan; Halsey,Gregory; Satoskar,Abhay; Oghumu,Steve. |
| Abstract Neglected Tropical Diseases (NTDs) comprise of a group of seventeen infectious conditions endemic in many developing countries. Among these diseases are three of protozoan origin, namely leishmaniasis, Chagas disease, and African trypanosomiasis, caused by the parasites Leishmania spp., Trypanosoma cruzi, and Trypanosoma brucei respectively. These diseases have their own unique challenges which are associated with the development of effective prevention and treatment methods. Collectively, these parasitic diseases cause more deaths worldwide than all other NTDs combined. Moreover, many current therapies for these diseases are limited in their efficacy, possessing harmful or potentially fatal side effects at therapeutic doses. It is therefore... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmaniasis; Chagas; Trypanosomiasis; Nanoparticles; Liposomes; Nanotubes; Nanodiscs; Micelles. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100201 |
| |
|
|
| Mohamad,Ebtesam Abdelghany; Fahmy,Heba Mohamed. |
| The leaf extract of Annona squamosa L. has antibacterial, antidiabetic, antioxidant, and anticancerous activities. The present work aims to compare between liposomes and niosomes as carriers for A. squamosa extract to improve its transdermal bioavailability. Physical characterization for niosomes and liposomes was performed using: transmission electron microscope (TEM), scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR). In addition, the encapsulation efficiency for A. squamosa in both carriers was evaluated and in-vitro drug release experiments were performed. The results proved the potential of both carriers to penetrate the outer layer of the skin (stratum corneum) which is considered as a strong barrier against the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Annona squamosa; Dermal delivery; Liposomes; Niosomes. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100525 |
| |
|
| |
|
|
| Frézard,F.; Michalick,M.S.M.; Soares,C.F.; Demicheli,C.. |
| The antimonial drug, meglumine antimoniate, was successfully encapsulated in dehydration-rehydration vesicles and in freeze-dried empty liposomes (FDELs). High encapsulation efficiencies (from 28 to 58%) and low weight ratios of lipids to encapsulated antimony (from 1:0.15 to 1:0.3) were achieved. These formulations, contrary to those obtained by conventional methods, can be stored as intermediate lyophilized forms and reconstituted just before use. The efficacy of FDEL-encapsulated meglumine antimoniate was evaluated in hamsters experimentally infected with Leishmania chagasi. A significant reduction of liver parasite burdens was observed in animals treated with this preparation, when compared to control animals treated with empty liposomes. In contrast,... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Liposomes; Glucantime; Meglumine antimoniate; Leishmaniasis; Encapsulation. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700016 |
| |
|
| |
|
|
| Espinoza,Joel Toribio; Novak,Robson Schimandeiro; Magalhães,Cássia Gonçalves; Budel,Jane Manfron; Justus,Barbara; Gonçalves,Melissa Marques; Boscardin,Patricia Mathias Döll; Farago,Paulo Vitor; Paula,Josiane de Fátima Padiha De. |
| The objective of this work was to develop and characterize liposomes loaded with silver nanoparticles (LAgNPs) to show improvement in stability characteristics. AgNPs were prepared by the green synthesis method with Aloe vera gel extract and exposure to sunlight. Liposomes were prepared by the modified reverse phase method. Particle size, polydispersity index, zeta potential, as well as the scanning electron microscopy (SEM) morphological aspects of AgNPs and LAgNPs were evaluated. In addition, was used flame atomic absorption spectroscopy to determine the amount of AgNP that was encapsulated in liposomes. The AgNPs presented as amorphous and polydisperse structures, with a mean diameter of 278.46 nm and zeta potential of -18.3 mV. LAgNPs had a mean... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Silver nanoparticles; Aloe vera; Sunlight; Liposomes. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100625 |
| |
| Registros recuperados: 24 | |
|
|
|
